Figure 3. Local activation of influenza-specific CTLs in the lungs depends on TLR7 activation.

(A) NP_366–374 tetramer-positive CD8+ T cells in the lungs of wt (black bars) and TLR7−/− (white bars) mice vaccinated with WIV were quantified on day 6 post-challenge. Gating strategy was as described in the legend to Fig. 2 (B) Local production of granzyme B in the lungs of wt (black bars) and TLR7−/− (white bars) mice vaccinated with WIV was measured on day 6 post-challenge by ELISA performed on BAL samples. Bars represent mean±SEM of 3 mice per group. *p<0.05; Mann-Whitney U-test.