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. 2013 May 2;8(5):e63163. doi: 10.1371/journal.pone.0063163

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© 2013 Budimir et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Bone marrow-derived pDCs (B220+CD11c+CD11b−), cDCs (B220-CD11c+CD11b+) and unsorted DCs were pulsed for 24 hr with WIV (gray histogram) or culture medium (white histogram). Upregulation of surface maturation marker expression was measured by flow cytometry. Gates were set on viable cells based on the forward/side scatter profile and exclusion of 7AAD+ cells. Numbers above each histogram represent the mean fluorescence intensity measured in medium-stimulated and WIV-stimulated DCs, respectively. Results are representative of 3 independent experiments.