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. 2013 May 2;9(5):e1003331. doi: 10.1371/journal.ppat.1003331

Figure 4. TgNST1 deletion interferes with recognition by the DBA and WGA lectins.

Figure 4

WT, Δnst1, or Δnst1:NST1 parasites were grown under bradyzoite-inducing conditions for 4 days in vitro, fixed in formaldehyde, permeabilized and stained using the FITC-conjugated lectins DBA (A) or WGA (B). Antibodies against the bradyzoite-specific antigen SRS9 and Alexa 647-conjugated goat anti-rabbit antisera (pseudocolor red) were use to identify bradyzoites within cysts. Scale bar represents 10 µm. C) Mice infected with the WT, Δnst1 and Δnst1:NST1 strains were sacrificed 3 weeks post-infection, perfused with heparine/saline and brains were drop-fixed in paraformaldehyde, sucrose-embedded and cryosectioned for immunohistochemistry. 40 µm sections were stained using the FITC-DBA lectin and antibody against the bradyzoite-protein SRS9, as in parts A and B. 1 µm slice images were taken using a Zeiss confocal microscope and a single slice is shown. Scale bar represents 10 µm.