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Mice were infected i.p. with 500 WT, Δnst1 or Δnst1:NST1 tachyzoites.12 days post-infection, animals were sacrificed and the posterior left quarter of the brain was used to quantify the number of parasites present by real-time quantitative PCR detection of Toxoplasma's B1 gene normalized to the detection levels of mouse actin (ACT1).
