Table 1. Molecular weights of the precursor and product ions−1 of the metabolites and their deuterated internal standards selectively monitored by LC-tandem MS.
Precursor | Product | Precursor | Product | ||
Analog | ion−1m/z | ion−1m/z | Analog | ion−1m/z | ion−1m/z |
PGE2 | 351 | 271 | d7-5-oxo-ETE | 324 | 210 |
d4-PGE2 a | 355 | 275 | LTB4 | 335 | 194.8 |
PGE3 | 349 | 269 | d4-LTB4 | 339.2 | 196.8 |
PGD2 | 351 | 271 | 15-HETE | 319 | 218.8 |
d4-PGD2 | 355 | 275 | d8-15-HETE | 327 | 225.7 |
5-HETE | 319 | 115 | 12-HETE | 319 | 179 |
d8-5-HETEb | 327 | 115.8 | d8-12-HETE | 327 | 184 |
5-HEPE | 317 | 115 | 13-HODE | 295 | 194.7 |
5-oxo-ETE | 317 | 203 | d4-13-HODE | 299 | 197.7 |
Internal standard for PGE2 and PGE3.
Internal standard for 5-HETE and 5-HEPE.