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. 2013 May 2;8(5):e63039. doi: 10.1371/journal.pone.0063039

Figure 5. Maturation of DCs induced by particle-bound PADRE is via sphingosine kinase but not PLC.

Figure 5

Effect of the PLC inhibitor D609 (A) and the sphingosine kinase inhibitor SKI-II (B) on the phenotypic maturation of DCs induced by particle-bound PADRE. Day-5 DCs were incubated with the inhibitors for 2 hours and then cultured with particle-bound PADRE, particle-bound P2C or ionomycin for 2 days and analyzed by flow cytometry for CD80, CD86, CD83 and HLA-DR. The histograms show the expression levels of a representative donor of at least three independent experiments done with cells from three different healthy donors.