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. 2013 May 2;8(5):e63039. doi: 10.1371/journal.pone.0063039

Figure 8. Effect of the specific inhibitors of kinase signaling on the phenotypic maturation of DCs induced by particle-bound PADRE.

Figure 8

Day-5 DCs were incubated for 2 hours with inhibitors for p72 Syk, PI3K, p38 MAPK or NF-κB after which particle-bound PADRE, particle-bound P2C or soluble LPS were added and the cultures continued for 2 more days. On day 7 the cells were analyzed by flow cytometry for expression of CD80, CD86, CD83 and HLA-DR and their culture supernatant by ELISA for IL-12p70. The graphs show fold change of expression of CD80 (A), CD86 (B), CD83 (C) and HLA-DR (D), or IL-12p70 levels in the supernatants (E) with standard error of 7 independent experiments with cells from 7 different healthy donors. * = p<0.05; ** = p<0.01; and *** = p<0.001 comparing the group treated with stimulus only vs. the groups treated with stimulus plus the specific inhibitor.