Table 1. Relative expression levels of GCDH in different groups as detected by RT-PCR.

	△Ct(GCDH-GAPDH)	Relative expression
control	7.719±0.1233	1.129
NC	7.893±0.2401	1
Lentivirus-shRNA#1	9.343±0.0306*	0.366
Lentivirus-shRNA#2	9.020±0.0100*	0.458
Lentivirus-shRNA#3	9.253±0.0153*	0.390

^* P<0.05 vs. NC group. There were no significant differences between the control and NC group with respect to the level of GCDH mRNA level. The mRNA levels of GCDH in lentivirus-shRNA#1, 2, and 3 subgroups were reduced by 63.4%, 54.2%, and 61.0%, respectively.