Figure 1.

Diagrammatic representation of the regulatory sites surrounding the human AR promoter and 5′-UTR identified by computer program Gene-Runner. pLARS-1, pAR-Sp1-3-O and its mutant pMAR-Sp1-3-O are AR-luciferase reporters constructed in our laboratory and were used for in this study. pSp1-luc and pmSp1-luc used in this study are plasmids containing three tandem repeats of consensus Sp1 sites and their corresponding mutant that drive luciferase gene (kindly provided by Dr. Yoshihiro Sowa, University of Medicine, Kamigyo-ku, Kyoto, 602, Japan.).