Fig. 2.

Physical association of Tespa1 with IP₃R subtypes. (A) Immunoprecipitations with anti-Tespa1 (αTespa1) or control (preimmune) antibodies were performed using the mouse thymus or spleen, followed by Western blotting with anti-Tespa1, anti-IP₃R1, or anti-IP₃R3 antibodies. (B) Immunoprecipitations with IP₃R3 (αIP₃R3) or control (Ctl.IgG) antibodies were performed using the mouse thymus or spleen, followed by Western blotting with anti-Tespa1 or anti-IP₃R1 antibodies. (C and D) Immunoprecipitations with anti-Tespa1 (αTespa1) or control (preimmune) antibodies were performed using B220⁺–B lymphocytes or CD4⁺–T lymphocytes isolated from the adult mouse spleen (C), or using T-cell leukemia lines, Jurkat or EL4 (D), followed by Western blotting with anti-Tespa1, anti-IP₃R1, or anti-IP₃R3 antibodies. IP, Immunoprecipitations; total, total lysate. (E) Confocal immunostaining with anti-Tespa1 and anti-IP₃R3 antibodies in Jurkat cells. Blue, 4′,6-Diamidino-2-phenylindole (DAPI) staining. Scale bar, 15 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)