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. 2012 Sep 4;2:267–272. doi: 10.1016/j.fob.2012.08.006

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© 2012 Published by Elsevier B.V. on behalf of Federation of European Biochemical Societies.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non- commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig. 1 — Separation of PA and identification of PA molecular species by LC/ESI-MS. (A) PAs in MEFs were separated from other phospholipids by the Accela LC System using a UK-Silica column with mobile phases containing 0.28% ammonia. (B) Negative-ion ESI/MS spectra with PA species from m/z 591.41 (28:0-PA) to m/z 759.59 (40:0-PA) were identified. Abbreviations:I.S., internal standard, PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; SM, sphingomyelin.