Figure 4. qRT-PCR of four transcripts upregulated upon T. cruzi infection.

Amounts of mRNA from nitrophorin-like protein (A), 14 kDa protein (B), lysozyme (C) and cathepsin D (D) were obtained by relative quantification. Adult insects infected with T. cruzi and uninfected T. infestans were used for analysis (three biological samples were used for both the uninfected and infected groups). All data were normalized to 18S ribosomal RNA, representing the mean (n = 3) of identical triplicates ± standard deviation. An unpaired t test was performed for statistical analysis, and differences were considered significant at P<0.05. Asterisks represent significant differences (** P<0.01; *** P<0.001).