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. 2013 May 2;8(5):e62934. doi: 10.1371/journal.pone.0062934

Figure 5. Down-regulation of GPR109A in human macrophage-derived foam cells.

Figure 5

5A GPR109A mRNA was not expressed in undifferentiated THP-1 cells but expression was seen after differentiation into basal macrophages. Treatment with NA for 24 hours caused significant down-regulation of GPR109A mRNA transcription (n = 3 per group). Foam cells induction down-regulated GPR109A mRNA transcription compared to basal macrophages (n = 3 per group). 5B Western blot showed the loss of protein expression of GPR109A in total cell lysates from foam cells compared to basal macrophages. 5C and 5D Confocal fluorescence immunocytochemistry demonstrated the expression of GPR109A on the cell surface in cultured THP-1 basal macrophages (5C), and the down-regulation of GPR109A expression after foam cell induction (5D).