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. 2013 May 2;8(5):e62912. doi: 10.1371/journal.pone.0062912

Figure 1. Detection of S-ASM activity in CSF and linear relationship between product, sample volume and time.

Figure 1

A: Separation of reaction mixtures by thin layer chromatography with the fluorescent substrate BODIPY-C12-SM at the application site and the migrated fluorescent product, BODIPY-C12-ceramide, for samples of plasma (P), serum (S), tissue lysate (T), CSF in addition to heat-denatured CSF (dCSF) and water (N) as the negative control as well as ceramide (Cer) and dodecanoic acid (FA) as reference. B: Linear correlation between the amount of generated product and the applied volume. C: Linear correlation between the amount of generated product and the reaction time.