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. 2013 May 2;8(5):e62912. doi: 10.1371/journal.pone.0062912

Figure 2. Optimization of reaction conditions for S-ASM activity in CSF.

Figure 2

A: Activity in a series of Carmody buffers of varying pH. B: Broad optimal range for NaCl concentrations. C: Strong influence of the type and concentration of detergent (SDS, sodium dodecyl sulfate; NaDOC, sodium deoxycholate). D: Strong influence of the type and concentration of detergent on S-ASM originating from plasma for comparison. E: Dependence of S-ASM activity in CSF on the concentration of Nonidet P-40 detergent in the presence of additional bovine SM or denatured plasma (dP). F: Comparison of the effect of the detergent concentration on S-ASM from CSF and plasma in the presence of denatured plasma (dP) or CSF (dCSF), respectively. G: S-ASM dependence on addition of zinc ions. H: Very low residual S-ASM activity following addition of various amounts of EDTA.