Figure 3. Michaelis-Menten kinetics for the reaction of S-ASM in CSF with an optimum at 37°C.

A: Influence of incubation temperature on S-ASM activity in CSF. B: Dependence of S-ASM activity on the concentration of the substrate BODIPY-C12-SM under constant concentrations of DMSO (introduced by the substrate stock). C: Lineweaver-Burk plot with the line graph calculated by nonlinear regression.