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. 2013 May 2;8(5):e56580. doi: 10.1371/journal.pone.0056580

Figure 3. Compression caused apoptosis in spinal cord cells.

Figure 3

(A) The apoptosis of cells in spinal cord (SC), cauda equina (CE) and dorsal root ganglion (DRG) were analyzed by TUNEL method in three groups: before surgery; sham-operated; MCC group. (B) TUNEL positive cells were counted in >5 fields and the average numbers were shown in different groups. (C) qRT-PCR analysis of the expression of Bcl-2, Bcl-xl, Bax, Bak and Bad in three experimental groups. (D) The expression level of pro-caspase-3 (pro-casp-3) and active caspase-3 (active caspase-3) was determined by western blot in indicated 3 groups.