Table III.
Linear models with methylation level as the response, and smoking, age, gende, HPV status and tumor site as the covariates. Smoking is coded as continuous variable: 1=”never smoked”, 2=”pipe or cigar only”, 3=”cigarette: <20 pack-years”, 4=”cigarette: 20–40 pack-years”, 5=”cigarette: >40 pack-years”. Seven subjects had unkown smoking history and were excluded from this analysis, leaving 346 subjects in this analysis. HPV status was imputed to be negative for samples from sites other than oropharynx.
| P16 | |||
| Estimate | 95% CI | P-value | |
| Intercept | 15.514 | (−0.254, 31.282) | 0.054 |
| Smoking | −1.818 | (−3.705, 0.069) | 0.059 |
| Age | 0.076 | (−0.135, 0.286) | 0.480 |
| Gender: female | |||
| male | 2.886 | (−3.472, 9.244) | 0.373 |
| HPV: no | |||
| yes | −5.022 | (−17.960, 7.916) | 0.446 |
| unkown | −8.062 | (−26.408, 10.284) | 0.388 |
| Site: oral cavity | |||
| oropharynx | −5.574 | (−17.038, 5.890) | 0.340 |
| hypopharynx | −13.374 | (−24.780, −1.967) | 0.022 |
| larynx | −10.779 | (−18.163, −3.394) | 0.004 |
| other | 2.039 | (−10.407, 14.485) | 0.747 |
| multiple | 17.271 | (−11.786, 46.329) | 0.243 |
| DCC | |||
| Estimate | 95% CI | P-value | |
| Intercept | 4.503 | (−12.572, 21.578) | 0.604 |
| Smoking | −1.787 | (−3.830, 0.257) | 0.086 |
| Age | 0.253 | (0.025, 0.481) | 0.030 |
| Gender: female | |||
| male | 7.006 | (0.122, 13.891) | 0.046 |
| HPV: no | |||
| yes | 14.791 | (0.781, 28.801) | 0.039 |
| unkown | 9.896 | (−9.971, 29.762) | 0.328 |
| Site: oral cavity | |||
| oropharynx | −5.745 | (−18.159, 6.670) | 0.363 |
| hypopharynx | 3.556 | (−8.796, 15.908) | 0.572 |
| larynx | −6.643 | (−14.640, 1.353) | 0.103 |
| other | 0.364 | (−13.113, 13.842) | 0.958 |
| multiple | −9.684 | (−41.150, 21.783) | 0.545 |
| EDNRB | |||
| Estimate | 95% CI | P-value | |
| Intercept | 13.489 | (−3.440, 30.418) | 0.118 |
| Smoking | −2.208 | (−4.234, −0.182) | 0.033 |
| Age | 0.287 | (0.061, 0.513) | 0.013 |
| Gender: female | |||
| male | 1.321 | (−5.504, 8.147) | 0.704 |
| HPV: no | |||
| yes | 0.717 | (−13.174, 14.607) | 0.919 |
| unkown | 1.678 | (−18.019, 21.375) | 0.867 |
| Site: oral cavity | |||
| oropharynx | −0.594 | (−12.902, 11.715) | 0.924 |
| hypopharynx | −3.062 | (−15.309, 9.185) | 0.623 |
| larynx | −7.457 | (−15.385, 0.472) | 0.065 |
| other | −0.095 | (−13.458, 13.267) | 0.989 |
| multiple | −18.193 | (−49.390, 13.005) | 0.252 |
| KIF1A | |||
| Estimate | 95% CI | P-value | |
| Intercept | 11.429 | (−2.753, 25.611) | 0.114 |
| Smoking | −2.452 | (−4.149, −0.755) | 0.005 |
| Age | 0.243 | (0.053, 0.432) | 0.012 |
| Gender: female | |||
| male | 1.504 | (−4.214, 7.222) | 0.605 |
| HPV: no | |||
| yes | 4.704 | (−6.933, 16.340) | 0.427 |
| unkown | −3.572 | (−20.072, 12.928) | 0.671 |
| site: oral cavity | |||
| oropharynx | −7.934 | (−18.245, 2.377) | 0.131 |
| hypopharynx | −12.198 | (−22.457, −1.938) | 0.020 |
| larynx | −10.254 | (−16.896, −3.613) | 0.003 |
| other | −8.892 | (−20.085, 2.302) | 0.119 |
| multiple | −1.839 | (−27.973, 24.296) | 0.890 |