. Author manuscript; available in PMC: 2014 Jun 1.

Published in final edited form as: Biochim Biophys Acta. 2013 Mar 5;1831(6):1052–1059. doi: 10.1016/j.bbalip.2013.02.009

Table 4.

Fluorescence characterization of tryptophan mutants^a

Colipase	Buffer			NaTDC			NaTDC & Oleic Acid

	λ_MAX	Peak	I₃₃₀/I₃₅₀	Peak Shift	Peak	I₃₃₀/I₃₅₀	Peak Shift	Peak	I₃₃₀/I₃₅₀

	nm	Intensity		nm	Intensity		nm	Intensity
L34W	350	10.1	0.67	-14	19.8	1.17	-10	10.7	1.03
L36W	349	4.4	0.70	-6	8.6	0.93	-6	9.2	0.90
L54W	347	3.9	0.77	-3	5.0	0.90	-3	4.0	0.85
Y55W	350	4.3	0.63	-6	6.6	0.90	-19	5.3	1.23
I57W	347	4.7	0.75	-4	6.9	0.94	-4	5.3	0.93
Y59W	345	9.7	0.81	-2	9.8	0.96	-2	6.0	0.89
I75W	349	5.8	0.70	-3	6.8	0.80	-5	5.5	0.90
V76W	346	10.2	0.81	-3	10.3	0.91	-5	6.9	0.98
I79W	350	3.7	0.64	-4	5.2	0.83	-7	3.9	0.91
F84W	345	5.7	0.84	0	5.4	0.87	0	3.8	0.86

Fluorescence measurements were done in 0.02 M Tris-Cl, pH 8.0 as described in Experimental Procedures. When added, NaTDC was 4 mM and oleic acid was 4 mM. Excitation was at 295 nm. Emission was scanned from 300 to 450 nm. The excitation slit was set at 4 nm and the emission slit at 10 nm. Each scan was done 5 times and the results determined from the average fluorescence curve of those scans.