Fig. 5.

Immunohistochemical features of the surgical specimen. a Atypical large lymphoid cell in the intra-osseous area is positive for CD20 (original magnification ×200). b Atypical large lymphoid cell in the intra-osseous area is positive for CD30 (original magnification ×200). c EBV-LMP-1 positive cells, including Hodgkin and Reed-Sternberg-like cells, were present in multiple foci of granulomatous tissue surrounding severely necrotic foci (original magnification ×200). d The nuclei of large atypical cells are markedly positive for Ki-67 (original magnification ×200). e In situ hybridization for EBV shows large lymphoblastic cells are strong staining in the nuclei of Hodgkin or Reed-Sternberg-like giant cells (original magnification ×400)