MECT1-MAML2 fusion transcripts in MEC. a Gel electrophoresis from second nested RT-PCR reaction. a–f represent MEC. MEC in lanes A, C, and E demonstrate the MECT1-MAML2 fusion transcripts, at 117 bp, while the samples in lanes B, D, and F are negative. Lane G represents the positive control: MEC with MECT1-MAML2 fusion transcripts from a previous reaction, which was confirmed by sequencing. The negative controls are lane H (no template) and lane I (squamous cell carcinoma). Bottom Panels The MEC in panelB is negative for fusion transcripts; the reaction product did not migrate at 117 bp and the nucleotide sequences are different from those seen in panels C and D. The MEC in panels C and D are positive for the MECT1-MAML2 fusion transcript by RT-PCR. DNA nucleotide amplicon sequencing confirmed the presence of the t(11;19) CRTC1/MAML2 fusion gene :CGG*CTCCAGGGTTCCTTGAAAA (* is the breakpoint)