Table 1.

Primer designs for detection of HPV16/18 E6 and E7 cDNA for mucoepidermoid carcinoma

Primer	Reaction	HPV genome	Amplicon (bp)	Primer (bp)	Primer sequence
HPV18E6 FP	First PCR	331	250	24	5′-GAG AAT TAA GAC ATT ATT CAG ACT-3′
HPV18E6 RP	First PCR	581		24	5′-TTA TAC TTG TGT TTC TCT GCG TCG-3′
HPV18E6 FP	Nested real time PCR	428	142	24	5′-CCA GAA ACC GTT GAA TCC AGC AGA-3′
HPV18E6 RP	Nested real time PCR	570		24	5′-TTT CTC TGC GTC GTT GGA GTC GTT-3′
HPV18E7 FP	First PCR	644	240	24	5′-AAT GAA ATT CCG GTT GAC CTT CTA-3′
HPV18E7 RP	First PCR	884		24	5′-GAC ACA CAA AGG ACA GGG TGT TCA-3′
HPV18E7 FP	Nested real time PCR	725	135	24	5′-CAT CAA CAT TTA CCA GCC CGA CGA-3′
HPV18E7 RP	Nested real time PCR	860		24	5′-GAA ACA GCT GCT GGA ATG CTC GAA-3′
HPV16E6 FP	First PCR	299	257	24	5′-TGT TTA AAG TTT TAT TCT AAA ATT-3′
HPV16E6 RP	First PCR	556		24	5′-CAG CTG GGT TTC TCT ACG TGT TCT-3′
HPV16E6 FP	Nested real time PCR	361	164	24	5′-AAC ATT AGA ACA GCA ATA CAA CAA-3′
HPV16E6 RP	Nested real time PCR	525		24	5′-CTG CAA CAA GAC ATA CAT CGA CCG-3′
HPV16E7 FP	First PCR	591	249	24	5′-ATA TAT GTT AGA TTT GCA ACC AGA-3′
HPV16E7 RP	First PCR	840		20	5′-GAT GGG GCA CAC AAT TCC TA-3′
HPV16E7 FP	Nested real time PCR	681	127	24	5′-TCC AGC TGG ACA AGC AGA ACC GGA-3′
HPV16E7 RP	Nested real time PCR	831		24	5′-GCA CAC AAT TCC TAG TGT GCC CAT-3′
GAPDH FP	PCR	1003	179	24	5′-ACT GAG CAC CAG GTG GTC TCC TCT-3′
GAPDH RP	PCR	1182		20	5′-TTA CTC CTT GGA GGC CAT GT-3′