FIGURE 3.

Effect of T-type Ca²⁺ channels modulation on the [Ca²⁺]_cyt elevations evoked by OGD. A, [Ca²⁺]_cyt recordings during 90 min of OGD. The delayed [Ca²⁺]_cyt elevation was reduced by Ni²⁺ and Cav3.1/Cav3.2 silencing and increased by Cav3.2 overexpression. Traces are the averaged [Ca²⁺]_cyt responses measured in the indicated conditions. B, [Ca²⁺]_cyt elevations evoked by 30 min of OGD. External Ca²⁺ removal prevented the elevations. C, basal [Ca²⁺]_cyt levels recorded under the different conditions. D, amplitude of the initial [Ca²⁺]_cyt elevation evoked by OGD. Data are mean ± S.E. from six to eight independent recordings. The total number of measured cells is indicated inside the bars. E, effect of T-type Ca²⁺ channel modulation on the slope of the secondary [Ca²⁺]_cyt elevation. Data are mean ± S.E. from three to four independent recordings, expressed as fold changes from the control condition. The total number of measured cells is indicated inside the bars. F, cytotoxicity as a function of the Ca²⁺ slope measured in the indicated conditions. Data are from Figs. 2A and 3E. ns, non-significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001 (Student's t test for unpaired samples).