FIGURE 6.

Effect of T-type Ca²⁺ channel activation on [Ca²⁺]_mit and [Ca²⁺]_cyt. A, [Ca²⁺]_mit responses evoked by the addition of 20 mm K⁺ to naïve and Cav3.1/Cav3.2-depleted cells. Cav3.1/Cav3.2 silencing significantly decreased the amplitude of the [Ca²⁺]_mit response. B, [Ca²⁺]_cyt responses recorded under the same conditions. C, [Ca²⁺]_mit responses evoked by the addition of 100 μm ATP to evoke rapid Ca²⁺ release from intracellular stores. D, [Ca²⁺]_mit responses evoked by the addition of thapsigargin followed by Ca²⁺ readmission to promote Ca²⁺ entry across store-operated Ca²⁺ entry channels. Cav3.1/Cav3.2 silencing had no effect on the mitochondrial Ca²⁺ responses associated with ER Ca²⁺ release or store-operated Ca²⁺ entry-mediated Ca²⁺ influx. Data are mean ± S.E. from three to six independent recordings. The total number of measured cells is indicated inside the bars. ns, non-significant; **, p < 0.01 (Student's t test for unpaired samples).