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. 2013 Mar 15;288(18):12469–12477. doi: 10.1074/jbc.M112.433979

FIGURE 3.

FIGURE 3.

BiFC analysis comparing Tie2 and Tie2 mutant. A, detection of Tie2, kinase-dead mutant Tie2K854R (K/R) and constitutively active mutant Tie2R848W (R/W) phosphorylation. B and C, HEK293T cells were transiently transfected in combination with Tie2-HAVN and Tie2-MycVC, Tie2K854R-HAVN and Tie2K854R-MycVC, or Tie2R848W-HAVN and Tie2R848W-MycVC. B, cells were analyzed by confocal microscopy. Bar indicates 20 μm. C, quantitative evaluation of receptor dimerization in BiFC as shown in B (*, p < 0.05; n = 3). Protein expression level of each receptor was assessed by immunoblotting with anti-HA or anti-Myc Ab. D, quantitative evaluation of receptor dimerization in BiFC of Tie2 and Tie2R848W (*, p < 0.05; n = 3). Protein expression level of each receptor was assessed by immunoblotting with anti-Tie2. N.S., not significant.