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. 2013 Mar 15;288(18):12469–12477. doi: 10.1074/jbc.M112.433979

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Tie2YIA/LAS cannot form ligand-independent dimers but is dimerized and phosphorylated upon stimulation with Ang1. A, dimerization and localization of Tie2 were observed in Tie2-VN- and Tie2-VC-coexpressing NIH3T3 cells in the presence or absence of Ang1. Wild-type Tie2 can form dimers irrespective of Ang1 stimulation, as confirmed by BiFC. However, this dimerized Tie2 forms cluster-like aggregations and is internalized upon stimulation with Ang1. B, similar to A, dimerization and localization of Tie2YIA/LAS (Tie2LAS) is observed in Tie2YIA/LAS-VN- and Tie2YIA/LAS-VC-coexpressing NIH3T3 cells. In the absence of Ang1, Tie2LAS did not dimerize but formed cluster-like aggregations upon stimulation with Ang1. Bar indicates 20 μm.