TABLE 1.

Kinetic parameters of MarP_179–397

Protease activity assays were performed as described under “Experimental Procedures.”

Substrate	Kma	Vmaxa	kcat	kcat/Km
	μm	pm s−1	s−1	m−1 s−1
WKLL-AMC	42.62 ± 4.4	145.6 ± 3.5	5.8 × 10−4 ± 1.4 × 10−5	13.7 ± 1.1
AMC-PSLIAKWV-K(DNP)	6.5 ± 0.6	806.5 ± 18.3	4.0 × 10−4 ± 7.3 × 10−5	62.0 ± 12.4

^a Parameters were determined by non-linear curve fitting of the data to the Michaelis-Menten equation.