TABLE 2.
Effect of protease inhibitors on MarP_179–397 mediated hydrolysis of WKLL-AMC
Inhibition of protease activity was determined as described under “Experimental Procedures.” Samples were run in triplicate. nd, not determined.
| Drug | Concentration | Target | % Inhibition | IC50 |
|---|---|---|---|---|
| μm | ||||
| Pepstatin A | 20 μm | Aspartic acid proteases | 26.4 | nd |
| PMSF | 1 mm | Serine proteases | 100 | 284 ± 14 |
| E-64 | 2 μm | Cysteine proteases | 23.8 | nd |
| EDTA | 10 mm | Metalloproteases | 8.0 | nd |
| Sodium fluoride | 20 mm | Serine/threonine and acidic phosphatases | 12.2 | nd |
| Fluorophosphonate-rhodamine | 20 μm | Serine proteases | 94.8 | 4.48 ± 0.23 |
| DTT | 2 mm | Reduces disulfides | 97.5 | nd |