FIGURE 1.

IMP3 promotes chemoresistance in triple-negative breast cancer cells. A and B, IMP3 was depleted using two shRNAs (shIMP3-1 and shIMP3-2) in SUM-1315 (A) and MDA-468 cells (B), and its expression was analyzed by immunoblotting and real-time PCR. shCtrl, short hairpin control. C–E, control (Ctrl, shRNA targeting GFP) and IMP3-depleted SUM-1315 (C–E, left panel) and MDA-468 cells (C–E, right panel) were treated with either vehicle or varying concentrations of doxorubicin, mitoxantrone, or taxol for 48 h, and cytotoxicity was measured by the MTT assay. The relative cell viability of the cells treated with vehicle was normalized to 100. Data presented are the mean of three independent experiments. Error bars in all panels indicate S.D. *, p ≤ 0.05.