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. 2013 Mar 28;288(18):12569–12573. doi: 10.1074/jbc.C112.442319

FIGURE 3.

FIGURE 3.

Restoration of BCRP expression in IMP3-depleted cells increases chemoresistance. A, IMP3-depleted SUM-1315 cells were infected with a lentivirus expressing BCRP, and its expression was determined by immunoblotting and real-time PCR. Sh-1+BCRP and sh-2+BCRP designate BCRP restoration in IMP3-depleted cells (shIMP3-1 and shIMP3-2). shCtrl, short hairpin control. B, control (Ctrl, shGFP), IMP3-depleted, and IMP3-depleted cells with restored BCRP expression were treated with either vehicle or varying concentrations of doxorubicin (left) and mitoxantrone (right), and cytotoxicity was measured using the MTT assay. C, SUM-1315 cells were infected with the lentivirus expressing BCRP and treated with vehicle or varying concentrations of doxorubicin and mitoxantrone. Cytotoxicity was measured by MTT assay. The immunoblot inside the graph (left) shows BCRP expression. Data are representative of three independent experiments. Error bars in all panels indicate S.D. *, p ≤ 0.05.