FIGURE 3.

DegB_DD did not alter the nuclear localization of GFP-DegAB. A, schematic presentation of Ura3-GFP-DegAB (UG-DegAB). B, degradation kinetics of UG-DegAB and UG-DegAB_DD in wild type and doa10Δ cells are shown. Proteins levels were measured using anti GFP antibodies. C, the cellular localization of the GFP fusion proteins was determined by fluorescence microscopy. Nuclear staining of the accumulated proteins was indicated by their co-localization with Pus1-RFP, a nuclear protein. No changes in the cellular localization of the various stabilized proteins were indicated.