FIGURE 5.

DegB_DD stabilized XBP1u and XBP1s expressed in HEK293T cells. Degradation of the indicated proteins, expressed from a pcDNA3 vector, was determined by pulse-chase analysis. Cells metabolically labeled with [³⁵S]methionine/cysteine were sampled at the indicated time periods, followed by lysis, and immunoprecipitation, as described under “Experimental Procedures.”