FIGURE 5.

Sdc4 and PKCα mediate PGE₂-induced in vivo angiogenesis. Matrigel containing either vehicle, PGE₂ (10 μm), or VEGF-A (100 ng/ml) was injected in the mouse flank area and excised 7 days later to evaluate angiogenesis. A, representative pictures of plugs extracted from WT, Sdc4^−/−, or PKCα^−/− mice. Ctr, control. B, quantification of Matrigel plug angiogenesis. Angiogenesis was evaluated by staining four different sections/plug and acquiring five random pictures in each section. Angiogenesis in each plug was calculated as mean of CD31-positive fluorescence pixels in the total pixel area. The diagram was generated by means derived from multiple plug experiments. Data are mean ± S.E., n = 4–12. C, explanation of the statistical symbols and p values in B.