FIGURE 7.

Expression of FLIP PTM mutants rescues PPC1 cells from paraquat-induced cell death and sensitization to TRAIL. A, PPC1 cells were treated with or without TRAIL (10 ng/ml) for 24 h. At various times, cells were lysed and ATP levels were measured using cell titer glow to assess cell viability. B, PPC1 cells were transfected with various HA-tagged FLIP plasmids (WT, T166A, K167R, and T166A,K167R double mutant) for 16 h and then treated with increasing concentrations of paraquat alone or (C) with 10 ng/ml of TRAIL for 12 h. Cells were then lysed and ATP levels were measured using cell titer glow to assess cell viability. Vector groups with no treatment were adjusted to 100%. Statistical significance (mean ± S.E.; n = 4) was determined by repeated measures analysis of variance and Tukey post-test. *, indicates p < 0.01 for a comparison with control PPC1 cells.