TABLE 1.
Assignments for peptide sequences identified from trypsin digestion of captured FLIP protein bands
The whole lane of c-FLIP protein bands were excised from the SDS-PAGE gel, reduced, alkylated, digested by trypsin, and subjected to LC/MS-MS analysis using the Michrom Paradigm MS4 HPLC.HTC-PAL autosampler/LTQ Orbitrap XL mass spectrometery for the analyses. The first column indicates the number of the first and last amino acid of the identified FLIP peptides, whereas the second column shows the corresponding amino acid sequences. TP or T(181 amu) indicates Thr mass + phospho group; KUb or K(242 amu) indicates Lys mass + ubiquitin GG tag; and MO or M(147 amu) indicates Met mass + oxidation.
| Residues | Sequence |
|---|---|
| 27–38 | DVAIDVVPPNVR |
| 39–45 | DLLDILR |
| 50–61 | LSVGDLAELLYR |
| 77–85 | KAVETHLLR |
| 95–106 | VLMOAEIGEDLDK |
| 141–154 | LNLVAPDQLDLLEK |
| 162–170 | IDLKTpKUbIQK |
| 213–226 | LKEQLGAQQEPVKK |
| 227–243 | SIQESEAFLPQSIPEER |
| 269–280 | DTFTSLGYEVQK |