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High performance anion exchange chromatography; chromatogram showing soluble aldonic acids (degree of polymerization 2–6) obtained upon incubation of 0.1% (w/v) PASC or 0.5% (w/v) steam-exploded spruce with 34 μg/ml PchGH61D in 25 μm sodium acetate, pH 5.3, 1.5 mm ascorbic acid for 20 h at 50 °C. Note the difference between the relative amounts of products released from the two substrates. Control reactions without enzyme yielded no detectable aldonic acids (data not shown).
