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Effect of H2O2 on TRPC6 mRNA expression in human MCs. Cells were without (time 0) or with H2O2 treatment at 200 μm for 1, 2, and 4 h in FBS-free medium. The mRNA level of TRPC6 was determined by real-time RT-PCR and normalized by β-actin mRNA levels. * denotes p < 0.05, compared with time 0, and † denotes p < 0.05, compared with 1 h. n indicates the number of independent experiments.
