FIGURE 7.

L-IDE expression modulates the RCR in HEKSw cells. A, increment in cellular mitochondrial content after PQQ exposure detected after 10-N-nonyl acridine orange staining and flow cytometry assay. Representative histograms (cell counts at each fluorescence intensity, 10¹–10²) for control (white) and PQQ (black) are shown. Significant differences were detected in fluorescence values between control and PQQ-treated cells (mean values in arbitrary units 28.74 ± 0.20 versus 30.31 ± 0.23; p < 0.001). B, mitochondrial respiration was determined by oxygen electrode measurement in HEKSw cells exposed or not to PQQ and treated with control siRNA (si-RNA-CNT) or IDE siRNA. *, p < 0.05, control (CNT) versus PQQ and control siRNA versus IDE siRNA. Inset, mean ± S.E. of state 4, state 3, and RCR (n = 5). Error bars represent S.E.