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Has2- and 4MU-mediated loss of hyaluronan inhibits myogenic differentiation. A, desmin immunofluorescence for fused cultures (24 h) following both control and Has2 siRNA transfection and the quantitated fusion index on the right. B, creatine kinase activity demonstrating reduced differentiation with Has2 siRNA. A greater inhibition of differentiation was observed with 4MU treatment by fusion index (C) and creatine kinase activity (D). *, p < 0.05, n = 4. Error bars, S.E.
