FIGURE 7.

Inhibition of differentiation by blocking hyaluronan synthesis cannot be rescued by restoration of the pericellular matrix. A, creatine kinase activity in C2C12 cultures that were incubated with hyaluronan-containing medium prior to and during differentiation show a minor reduction in differentiation. B, the addition of hyaluronan does not increase creatine kinase activity in 4MU-treated cultures 24 h after differentiation. C, the addition of hyaluronan does not increase creatine kinase activity in Has2 siRNA-treated cultures 24 h after differentiation. D, hyaluronan addition in conjunction with versican expression does not restore creatine kinase levels to control 24 h after differentiation. E, hyaluronan addition in conjunction with versican expression restores the erythrocyte-excluding pericellular matrix to 4MU hyaluronan synthesis-blocked cells, but neither hyaluronan nor versican could achieve this independently. *, p < 0.05, n = 4. Error bars, S.E.