FIGURE 6.

Culturing cells in the presence of EDTA disrupts colocalization of annexin 2 and DPP. Immunofluorescence rat ureteric bud cells grown in the presence of 1 mm EDTA is shown. A, DAPI staining to visualize the nuclei. B and C, RUB1 cells were grown on chamber slides in the presence of 1 mm EDTA, fixed, permeabilized, and reacted with anti-annexin 2 (B) and anti-DPP (C) antibodies. D, merging the annexin 2 and DPP images indicates that there is no colocalization between DPP and annexin 2 at the cell plasma membrane. Bar, 100 μm.