FIGURE 8.
Incubation of cells with anti-DPP lowers calcium uptake. Calcium uptake in RUB1 cells is shown. [Ca 2+]i was measured as described under “Experimental Procedures.” RUB1 cells were loaded with Fluo-3/AM, washed, and suspended in calcium- and magnesium-free flux buffer and then stimulated with 5 μm ionomycin and 2 mm calcium at the time points indicated. A shows calcium influx by cells alone (black trace) and cells treated with nonspecific IgG (red trace). B shows calcium influx by cells alone (black trace) and cells treated with anti-DPP (red trace).