FIGURE 1.

In postconfluent endothelial cells, Dlg1 is not localized at sites of cell-cell junctions. A and B, Dlg1 expression levels were compared by Western blotting in whole cell lysates of two different HUVEC cultures and of cultured hCMEC/D3 and Caco-2 cells. The same amounts of each postconfluent cell extract (20 μg protein) were loaded per lane. β-actin detection was used as a loading control. C–N, detection of the cell-cell junction markers E-cadherin (C), VE-cadherin (G and K), ZO-2 (D, H, and L), Scrib (E, I, and M) and Dlg1 (F, J, and N) by immunostaining of postconfluent Caco-2 cells, HUVECs, and hCMEC/D3. O and P, detection of Dlg1 by immunostaining of subconfluent Caco-2 cells and hCMEC/D3. Cells were imaged by epifluorescence microscopy. Scale bar = 10 μm. The white arrowheads indicate structures likely corresponding to the cytoskeleton.