FIGURE 2.

Bryo targets PKCα to the cell periphery and subsequently promotes temperature-dependent perinuclear accumulation of the enzyme. IEC-18 cells on glass coverslips were treated as follows: panel a, DMSO (Control) for 1 h at 37 °C; panel b, Bryo (100 nm) for 1 h at 37 °C; panel c, Bryo for 30 min at 37 °C followed by 2 h at 4 °C; panel d, DMSO for 2 h at 4 °C; panel e, Bryo for 2 h at 4 °C; panel f, Bryo for 2 h at 4 °C followed by 1 h at 37 °C. Cells were then fixed and processed for PKCα immunofluorescence. Arrows indicate perinuclear staining, and arrowheads point to membrane staining. Magnification bars, 10 μm. Data are representative of at least three independent experiments.