Figure 2.

Effect of Rab12 knockdown on mTORC1 activity. (A) Control and Rab12-knockdown MEFs were cultured under nutrient-rich or starved conditions, fixed and then immunostained with the antibodies indicated. Scale bar, 20 μm. (B) Control and Rab12-knockdown MEFs were cultured as in (A), and their lysates were analysed by immunoblotting with the antibodies indicated. (C) Lysates of MEFs that had been transfected with control or Rab12 siRNA were analysed by immunoblotting with the antibodies indicated. (D,E) Quantification of the phospho-S6K levels and phospho-Akt levels shown in (C). MEFs, mouse embryonic fibroblasts; N, nutrient-rich; S, starved; siRNA, short interfering RNA. *P<0.05; **P<0.01.