Figure 4.

PM–ER contacts affect PM stability in trans. (A–E) Yeast growth assays done in the absence (−) or presence of 0.1% NP-40 (+NP-40) on SD media containing choline. (F) Yeast growth assays done in the absence (−) or presence of cinnaymcin (+) on SD media containing choline. (G) Schematic of in vitro trans-methylation assay. Opi3 topology is on the basis of mammalian PEMT and boxes indicate regions containing catalytic sites [25]. (H) Distribution of label before and 120 min after mixing. (I) Distribution of label in the liposomal fraction 120 min after mixing. Error bars, s.d. ER, endoplasmic reticulum; PC, phosphatidylcholine; PDE, phosphatidyldimethylethanolamine; PM, plasma membrane; PME, phosphatidylmonomethylethanolamine; SD, synthetic-defined; WT, wild-type.