FIGURE 4.

Induction of macroautophagy in HT22 cells by methylene blue is mediated by AMPK pathway. (A) Representative Western blots showed that methylene blue increased AMPKα, pAMPKα, AMPKβ1, and pAMPKβ1 in a dose-dependent manner. Activating phosphorylation of ACC was also enhanced. (B,C) Representative Western blots demonstrated that 20 μM Compound C (Com C) significantly reduced methylene blue-induced ACC activation and LC3B activation in HT22 cells. Data are representative of three independent experiments.