Table 1.

Principal steps of the dissociation of morphologically intact glial cells (DIMIG).

1. Cortex dissection (p13–p15)

↓

2. Cortex slicing

↓

3. Pre-incubation of cortical tissue

↓

4. Enzymatic dissociation with papain

↓

5. Mechanical dissociation with pipettes

↓

6. One-step density gradient centrifugation

↓

7. Cytospin centrifugation

↓

8. Fixation of cells on slide