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. Author manuscript; available in PMC: 2013 May 3.
Published in final edited form as: Cancer Res. 2009 Jan 20;69(3):1143–1149. doi: 10.1158/0008-5472.CAN-08-3499

Table 1.

Mutation rates and spectra of tetranucleotide vectors in human lymphoblastoid cell lines

Class of mutation LCL721 (PMS2+) LCL1261 (PMS2−)
[TTCC/AAGG]9* [TTTC/AAAG]9 [TTCC/AAGG]9* [TTTC/AAAG]9
Microsatellite
  Expansions 12 (3, 5, 4) 12 (4, 5, 3) 42 (14, 16, 12) 34 (8, 17, 9)
  Deletions 14 (1, 8, 5) 9 (2, 4, 3) 2 (0, 2, 0) 8 (2, 1, 5)
  Other 1 (1, 0, 0) 0 0 0
Coding region† 6 (2, 0, 4) 10 (3, 2, 5) 10 (2, 4, 4) 11 (5, 5, 1)
Total 33 31 54 53
Median rate 13 × 10−6 37 × 10−6 140 × 10−6 170 × 10−6

NOTE: Mutation spectra of [TTCC/AAGG]9 and [TTTC/AAAG]9 were generated after replication in LCL721 and LCL1261. Numbers represent total number of occurrences, whereas those in parentheses indicate values for individual clones.

*

The difference in the ratio of expansions to deletions between [TTCC/AAGG]9 and [TTTC/AAAG]9 was statistically significant (P < 0.0001, Fisher’s exact test, 2-sided).

†

The difference between microsatellite and coding region mutations was not statistically significant between LCL721 and LCL1261 cell lines containing the same vector (P > 0.05, Fisher’s exact text, 2-sided).