Figure 5.

Structural model of the DNM1L oligomer. (A) DNM1L tetramers were manually fitted into the EM reconstruction of yeast Dnm1 (Mears et al, 2007). In this model, the stalks of DNM1L are oriented tangentially to the lipid tubule, with the B-insert pointing towards the tubule. Oligomerization of DNM1L into a filament proceeds via stalk interface-1, -2 and -3, as in dynamin and MxA. Additionally, stalk interface-4 mediates assembly of a double stalk filament. G domains could dimerize across neighbouring double filaments. (B) Schematic illustration of the DNM1L molecules in the oligomeric model. The repeating unit of the reconstruction (boxed) contains eight DNM1L monomers.